Mass Spectrometer¶
Peptide Mass Analysis¶
Category¶
Analysis Equipment
Application¶
Molecular weight confirmation and structural characterization
Function¶
Provides accurate molecular weight measurement of intact peptides and fragments.
Ionization Methods¶
| Method | Peptide MW Range | Features |
|---|---|---|
| ESI (standard) | 500–6000 Da | Most common, multiply charged |
| MALDI-TOF | 500–50,000 Da | High mass range, single charge |
| Direct infusion | 500–6000 Da | No LC coupling, fast analysis |
Analyzer Types¶
| Type | Resolution | Application |
|---|---|---|
| Quadrupole | Unit | Routine MS, quantitation |
| TOF (time-of-flight) | High | Accurate mass, intact MW |
| Ion trap | Unit | Fragmentation, structural analysis |
| Q-TOF | High | Accurate mass + fragmentation |
Key Specifications¶
| Parameter | Typical Value |
|---|---|
| Mass accuracy (TOF) | <5 ppm |
| Resolution (TOF) | 10,000–50,000 FWHM |
| Scan speed | >10 spectra/s |
| Sensitivity | femtomole level |
Related Tests¶
- Molecular weight confirmation
- Peptide mapping
-
Modifications analysis
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Peptide Mass Analysis — Review the section above for key parameters, methods, and quality criteria.
- Ionization Methods — Review the section above for key parameters, methods, and quality criteria.
- Key Specifications — Review the section above for key parameters, methods, and quality criteria.
Key Takeaways¶
- Peptide Mass Analysis — Review the section above for key parameters, methods, and quality criteria.
- Application — Review the section above for key parameters, methods, and quality criteria.
- Ionization Methods — Review the section above for key parameters, methods, and quality criteria.
- Analyzer Types — Review the section above for key parameters, methods, and quality criteria.
🔗 Related: LC-MS System | LC-MS Testing | Mass Confirmation