SPPS Workflow Diagram¶

Complete Solid-Phase Peptide Synthesis Process¶

flowchart TD
    A[Select Resin] --> B[Swelling<br/>DCM/DMF 30-60 min];
    B --> C[Load First<br/>Fmoc-AA-OH];
    C --> D[Cap Unreacted<br/>Sites];
    D --> E[Fmoc Deprotection<br/>20% Piperidine/DMF];
    E --> F[Wash × 4-6<br/>DMF];
    F --> G[Coupling<br/>Fmoc-AA-OH + Activator];
    G --> H[Wash × 3-4<br/>DMF];
    H --> I{Complete?};
    I -->|Kaiser Test+| J[Repeat Coupling];
    J --> H;
    I -->|Kaiser Test-| K[Next AA?];
    K -->|Yes| E;
    K -->|No| L[Final Fmoc<br/>Deprotection];

    L --> M[Wash × 5<br/>DCM, MeOH];
    M --> N[Cleavage Cocktail<br/>TFA/TIS/H2O];
    N --> O[Precipitation<br/>Cold Ether];
    O --> P[Centrifuge/Wash<br/>× 2-3];
    P --> Q[Dry under N2];
    Q --> R[Crude Peptide<br/>QC: HPLC, MS];

    style A fill:#e1f5fe
    style R fill:#e8f5e9
    style G fill:#fff3e0
    style N fill:#fce4ec

Cycle Description¶

Step	Key Action	Duration	QC Check
Resin selection	Choose based on C-terminal	—	Loading verification
Swelling	DCM or DMF	30–60 min	Visual
Loading	First AA attachment	1–2 h	Loading measurement
Capping	Ac₂O + DIEA	20–30 min	—
Deprotection	20% piperidine/DMF	10–15 min	UV 301 nm
Coupling	AA + activator + base	30–60 min	Kaiser test
Cleavage	TFA cocktail	2–4 h	—
Precipitation	Cold ether	5–15 min	Visual

Complete Solid-Phase Peptide Synthesis Process — Review the section above for key parameters, methods, and quality criteria.
Cycle Description — Review the section above for key parameters, methods, and quality criteria.

Key Takeaways¶

Complete Solid-Phase Peptide Synthesis Process — Review the section above for key parameters, methods, and quality criteria.
Cycle Description — Review the section above for key parameters, methods, and quality criteria.

🔗 Related: SPPS Process Detail | Manufacturing Workflow | Process Knowledge