Amino Acid Analysis¶
Peptide Composition Verification¶
Principle¶
Amino acid analysis (AAA) hydrolyzes the peptide into individual amino acids and quantifies each one, confirming the correct composition.
Method¶
- Hydrolysis: 6 M HCl, 110 °C, 24 hours
- Derivatization: Pre- or post-column
- Separation: HPLC or UPLC
- Detection: UV or fluorescence
What It Confirms¶
| Check | Purpose |
|---|---|
| Amino acid ratios | Match theoretical ratios |
| Absence of unexpected AAs | No contamination |
| Overall composition | Correct peptide |
What Are the Limitations of Amino Acid Analysis?¶
- Trp destroyed by acid hydrolysis
- Cys needs special derivatization
-
Asn/Gln converted to Asp/Glu
-
Peptide Composition Verification — Review the section above for key parameters, methods, and quality criteria.
- What It Confirms — See the section for detailed parameters and specifications.
- What Are the Limitations of Amino Acid Analysis? — See the section for detailed parameters and specifications.
Key Takeaways¶
- Peptide Composition Verification — Review the section above for key parameters, methods, and quality criteria.
- What It Confirms — See the section above for detailed parameters and specifications.
- What Are the Limitations of Amino Acid Analysis? — See the section above for detailed parameters and specifications.
🔗 Related: Mass Confirmation | HPLC Analysis