Acetonitrile¶
HPLC Mobile Phase Solvent¶
Definition¶
Acetonitrile (MeCN) is the primary organic solvent used as mobile phase B in peptide HPLC purification and analysis.
Specifications¶
| Parameter | Typical Value |
|---|---|
| Purity | ≥99.9% (HPLC grade) |
| UV cutoff | 190 nm |
| Water content | ≤0.02% |
| Grade | HPLC gradient grade |
Role in Peptide Analysis¶
| Function | Detail |
|---|---|
| Mobile phase B | Elutes peptides from C18 column |
| Gradient from 5–60% B | Separates peptide from impurities |
| Peptide solubility | Good for most peptides |
| UV transparency | Low UV absorption at 214 nm |
- HPLC Mobile Phase Solvent — Review the section above for key parameters, methods, and quality criteria.
- Role in Peptide Analysis — Review the section above for key parameters, methods, and quality criteria.
Key Takeaways¶
- HPLC Mobile Phase Solvent — Review the section above for key parameters, methods, and quality criteria.
- Definition — Review the section above for key parameters, methods, and quality criteria.
- Specifications — Review the section above for key parameters, methods, and quality criteria.
- Role in Peptide Analysis — Review the section above for key parameters, methods, and quality criteria.
🔗 Related: TFA | Preparative HPLC | HPLC Analysis