Primary SPPS Solvent
Definition
DMF (dimethylformamide) is the most common solvent in Fmoc-SPPS. It is used for resin swelling, coupling reactions, washes, and deprotection.
Specifications
| Parameter | Typical Value |
| Purity | ≥99.8% |
| Water content | ≤0.05% |
| Grade | Synthesis grade or HPLC grade |
Role in SPPS
| Function | Detail |
| Resin swelling | Swells polystyrene resin 4–6 mL/g |
| Coupling solvent | Dissolves Fmoc-amino acids and activators |
| Wash solvent | Removes excess reagents between steps |
| Deprotection | Carrier for piperidine (20%) |
Handling & Safety
| Hazard | Precaution |
| Toxic (teratogen) | Use fume hood |
| Absorbed through skin | Wear nitrile gloves |
| Flammable | Keep away from ignition sources |
| Hygroscopic | Seal tightly, use fresh |
- Primary SPPS Solvent — Review the section above for key parameters, methods, and quality criteria.
- Handling & Safety — Review the section above for key parameters, methods, and quality criteria.
Key Takeaways
- Primary SPPS Solvent — Review the section above for key parameters, methods, and quality criteria.
- Definition — Review the section above for key parameters, methods, and quality criteria.
- Specifications — Review the section above for key parameters, methods, and quality criteria.
- Role in SPPS — Review the section above for key parameters, methods, and quality criteria.
🔗 Related: DCM | Acetonitrile | Coupling Reaction