Oxyma — Coupling Additive¶
Safer Alternative to HOBt¶
Definition¶
Oxyma (ethyl 2-cyano-2-(hydroxyimino)acetate) is used as an additive with carbodiimide activators to suppress racemization and improve coupling efficiency.
Specifications¶
| Parameter | Typical Value |
|---|---|
| Purity | ≥99% |
| Appearance | White to off-white powder |
| Storage | 2–8 °C |
| CAS number | 3849-21-6 |
Advantages Over HOBt¶
| Feature | Oxyma | HOBt |
|---|---|---|
| Explosion hazard | None | Shock-sensitive at scale |
| Coupling efficiency | Comparable | Standard |
| Racemization suppression | Excellent | Good |
| Cost | Moderate | Low |
Typical Use¶
- With DIC (3 eq Oxyma + 3 eq DIC)
- In DMF or DMF/DCM mixtures
- 30–60 minute coupling time
Application in SPPS¶
Oxyma is widely adopted as the safer alternative to HOBt for large-scale and GMP peptide manufacturing.
- Safer Alternative to HOBt — Review the section above for key parameters, methods, and quality criteria.
- Advantages Over HOBt — Review the section above for key parameters, methods, and quality criteria.
- Application in SPPS — Review the section above for key parameters, methods, and quality criteria.
Key Takeaways¶
- Safer Alternative to HOBt — Review the section above for key parameters, methods, and quality criteria.
- Definition — Review the section above for key parameters, methods, and quality criteria.
- Specifications — Review the section above for key parameters, methods, and quality criteria.
- Advantages Over HOBt — Review the section above for key parameters, methods, and quality criteria.
🔗 Related: DIC | HBTU | Coupling Reaction